Journal
CHELONIAN CONSERVATION AND BIOLOGY
Volume 20, Issue 1, Pages 97-102Publisher
ALLEN PRESS INC
DOI: 10.2744/CCB-1387.1
Keywords
Vallarta Mud Turtle; cytochrome b; cytochrome oxidase I; mtDNA
Categories
Funding
- Turtle Conservation Fund
- Turtle Conservancy [5271.008-0272, TCF 0754]
- Global Wildlife Conservation
- [UDG-CA940]
- [UJAT-CA17]
Ask authors/readers for more resources
The Vallarta Mud Turtle, Kinosternon vogti, was described based on morphological characters, and further molecular data analysis supported its distinction as a unique lineage within the Kinosternon complex.
The Vallarta Mud Turtle (Kinosternon vogti) was recently described based solely on morphological characters; therefore, an examination of molecular data to determine the validity of this species is warranted. Here, mtDNA barcodes, phylogenetic trees, and three Operational Taxonomic Unit (OTU) analyses offer new evidence to support K. vogti as a distinct lineage within the Kinosternon complex. We generated 1,237 base pairs of the mitochondrial cytochrome oxidase I and cytochrome b genes from two paratype specimens collected at the species' type locality in Puerto Vallarta, Jalisco, Mexico, and compared them with 20 other sequences from BLAST belonging to close relatives representing 16 species. From these sequence data, we estimated genetic p-distances, reconstructed phylogenetic relationships among taxa, and performed 3 different operational taxonomic unit analyses (CD-HIT-EST DNA, ABGD, and Bayesian Poisson Tree Processes). The mean genetic p-distances over all sequence pairs was 0.083 +/- 0.00 substitutions per site, with the average number of base pair differences per site between K. vogti and all remaining sequences being 0.083 +/- 0.009. The closest species to K. vogti were K. hirtipes (p-distances 0.057 +/- 0.00) and K. scorpioides (p-distances 0.058 +/- 0.00). Phylogenetic trees from maximum likelihood and Bayesian analyses of the concatenated data set showed the same topology with generally high node support. The 3 operational taxonomic unit analyses identified K. vogti as a distinct OTU and likely a distinct evolutionary lineage.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available