Journal
CELLS TISSUES ORGANS
Volume 210, Issue 5-6, Pages 368-379Publisher
KARGER
DOI: 10.1159/000518667
Keywords
Glioma; Central nervous system; circRNA; miR-345; Hepatoma-derived growth factor
Funding
- Xiangya Hospital Central South University postdoctoral foundation
- Shenzhen Health Family Planning System Research Project [SZLY2017010]
- In hospital talent fund of Shenzhen Hospital Peking University [JCYJ2019001RC]
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Research has shown that hsa_circ_0073237 promotes the expression of HDGF in glioma cells by binding with miR-345, affecting the progression of tumors. The hsa_circ_0073237/miR-345/HDGF pathway may be a key target for treating glioma.
Glioma is the most common primary malignant tumor of the central nervous system and has a poor prognosis. Therefore, exploring the key molecular targets is a new opportunity for basic research and clinical treatment of glioma. Previous studies found that circRNA-hsa_circ_0073237 was upregulated in gliomas. Our further analyses of the biological function and molecular mechanism of hsa_circ_0073237 showed that hsa_circ_0073237 was also upregulated in glioma cell lines and could combine with miR-345 to inhibit its expression. miR-345 was downregulated in glioma tissues and cells, and targeted to regulate the expression of hepatoma-derived growth factor (HDGF), while HDGF expression was enhanced in glioma. Hsa_circ_0073237 promoted the expression of HDGF in glioma cells by adsorbing miR-345. Hsa_circ_0073237 siRNA, miR-345, and HDGF siRNA effectively inhibited cell viability and invasion and promoted cell apoptosis. When expression of hsa_circ_0073237 and miR-345 was inhibited simultaneously, cell viability, apoptosis, and invasion did not change significantly; however, after transfection with HDGF overexpression vector, the effects of hsa_circ_0073237 siRNA and miR-345 on glioma cell viability, apoptosis, and invasion were obviously reversed. Further construction of glioma xenograft models in nude mice confirmed that the introduction of miR-345 in vivo effectively inhibited tumor growth, significantly reduced tumor diameter and weight, and obviously decreased the expression of HDGF. Therefore, hsa_circ_0073237 can regulate the biological functions of glioma cells through miR-345/HDGF, thereby affecting the progression of tumors, indicating that the hsa_circ_0073237/miR-345/HDGF pathway may be a key target for the treatment of glioma. (C) 2021 S. Karger AG, Basel
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