Journal
CELL CYCLE
Volume 20, Issue 12, Pages 1163-1172Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2021.1929675
Keywords
Esophageal cancer; miR-2053; KIF3C; PI3K; AKT pathway
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Funding
- Henan Provincial People's Hospital & Medical Service Capacity Improvement Project in Henan Provincial Medical Institutions [23456]
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The study found that miR-2053 was downregulated in esophageal cancer cells, and its overexpression inhibited cell proliferation, migration, and invasion while promoting apoptosis, potentially through upregulating KIF3C expression and activating the PI3K/AKT signaling pathway in esophageal cancer development.
This study aimed to explore the role of micorRNA-2053 in esophageal cancer development. The expression level of miR-2053 in esophageal cancer cell lines was detected. After cell transfection, the effects of miR-2053 overexpression on proliferation, apoptosis, migration and invasion of esophageal cancer cells were determined. Moreover, the potential molecular mechanism was explored by measuring the epithelial-mesenchymal transition (EMT) and apoptosis-related proteins. Luciferase reporter assay was conducted to investigate the target gene of miR-2053. The protein expressions of PI3K/AKT pathway associated factors were detected after overexpression of miR-2053 or administration with the pathway inhibitor LY294002. The miR-2053 was downregulated in esophageal cancer cell lines. Overexpression of miR-2053 inhibited cell proliferation, migration and invasion while promoted apoptosis. Molecular mechanism elucidated that miR-2053 could reduce EMT and elevate the expression of pro-apoptotic proteins. Further study found that overexpressed miR-2053 could negatively regulate KIF3C and involve in PI3K/AKT signaling pathway. Our study demonstrated the downregulation of miR-2053 in esophageal cancer. Downregulation of miR-2053 involved in the proliferation, apoptosis, migration and invasion of esophageal cancer cells through upregulating KIF3C expression and activating the PI3K/AKT signaling pathway. miR-2053 may have the potential in clinical treatment of esophageal cancer.
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