Journal
CELL BIOCHEMISTRY AND FUNCTION
Volume 39, Issue 8, Pages 970-982Publisher
WILEY
DOI: 10.1002/cbf.3664
Keywords
activin B; activin/TGF-beta pathway; BMP pathway; dog; TGF-beta family
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Funding
- JSPS KAKENHI [18K05982]
- Grants-in-Aid for Scientific Research [18K05982] Funding Source: KAKEN
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The study characterized the canine inhibin beta B gene and its signaling pathways, identifying a long form of the gene that produces and secretes activin B, regulating the activin/TGF-beta and BMP pathways. The results provide important insights into canine diseases and reproductive functions.
Activin B, a homodimer of the inhibin beta B subunit, acts as a regulator of gonadal function and as an adipokine. To clarify the role of activin B in dogs, we characterized the canine inhibin beta B gene and signalling pathways regulated by the canine inhibin beta B. Using 5 '- and 3 '-rapid amplification of cDNA end (RACE) and RT-PCR on RNA isolated from the ovary of dogs, we identified short and long forms of the inhibin beta B gene. Immunoreactive inhibin beta B molecules were detected at similar to 25 and similar to 14 kDa under nonreducing and reducing conditions, respectively, in culture supernatants from HEK293 cells transfected with a plasmid containing the long form of the inhibin beta B gene, indicating activin B production and secretion. Similar to human and murine activin B, the canine activin B-stimulated transcriptions of reporter genes, CAGA-luc and Hepcidin-luc, regulated by the canonical activin/transforming growth factor-beta (TGF-beta) and bone morphogenetic protein (BMP) pathway, respectively. Activin B-induced CAGA-luc transcription was not detected in ALK7-deficient MDCK canine-derived cells; however, the forced expression of ALK7 resulted in the activin B-dependent expression in MDCK cells. Unexpectedly, the activin B-induced activation of the BMP pathway was partially blocked by the inhibition of endogenous activin/TGF-beta receptor activity. The present study identified an experimentally isolated long form of the canine inhibin beta B gene producing activin B that transactivates BMP- and activin/TGF-beta-regulated gene expression.
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