Journal
CARBON
Volume 179, Issue -, Pages 692-700Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.carbon.2021.04.061
Keywords
Carbon dots; Oxidase-like activity; Pyrrolic nitrogen; Visible light; Nanozyme
Funding
- National MCF Energy R&D Program of China [2018YFE0306105]
- National Key R&D Program of China [2020YFA0406104]
- Innovative Research Group Project of the National Natural Science Foundation of China [51821002]
- National Natural Science Foundation of China [51725204, 21771132, 51972216, 52041202]
- Natural Science Foundation of Jiangsu Province [BK20190041]
- Key-Area Research and Development Program of GuangDong Province [2019B010933001]
- Collaborative Innovation Center of Suzhou Nano Science Technology
- 111 Project
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Nitrogen-doped carbon dots (TA-NCDs) synthesized via hydrothermal method exhibit oxidase-like activity under visible light irradiation, with the potential to induce bioactivity in killing cells.
Carbon dots (CDs) with enzyme-like activities play crucial roles in nanobiotechnology. However, it is difficult to establish a relationship between their structure and biocatalytic properties. In this work, a series of nitrogen-doped carbon dots (TA-NCDs) were synthesized by a hydrothermal method. Their oxidase-like activity could be triggered by visible light irradiation (420 nm <=lambda <= 700 nm). As a nanozyme, TA-NCDs show the value of maximum reaction rates (V-max) is 121.95 x 10(-8) M/s and the Michaelis-Menten constants (K-m) is 0.61 mM. In situ transient photovoltage (TPV) tests prove that the pyrrolic nitrogen in TA-NCDs is the catalytic active sites and substrate binding sites. Under visible light irradiation, TA-NCDs, with oxidase-like activity, increased the cellular ROS level significantly, leading to their visible light induced bioactivity to kill nearly 60% of HeLa cells. (C) 2021 Elsevier Ltd. All rights reserved.
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