Journal
CARBOHYDRATE POLYMERS
Volume 264, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118059
Keywords
GH48 cellulase; Cellulose; Processivity; 3D-structure
Categories
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [14/00769-5, 15/13684-0]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [309506/2017-4, 303988/2016-9, 423693/2016-6]
- FAPESP [2013/08293-7, 2019/17350-0]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [14/00769-5] Funding Source: FAPESP
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Processive cellulases like BlCel48B from Bacillus licheniformis are efficient molecular engines that break down cellulose, with their enzymatic properties impacted by cellulose size and surface morphology. Structural analysis and molecular dynamics reveal the critical role of co-evolved residues in the active site in stabilizing ligands in the catalytic tunnel, providing mechanistic insights into activity and substrate binding of BlCel48B.
Processive cellulases are highly efficient molecular engines involved in the cellulose breakdown process. However, the mechanism that processive bacterial enzymes utilize to recruit and retain cellulose strands in the catalytic site remains poorly understood. Here, integrated enzymatic assays, protein crystallography and computational approaches were combined to study the enzymatic properties of the processive BlCel48B cellulase from Bacillus licheniformis. Hydrolytic efficiency, substrate binding affinity, cleavage patterns, and the apparent processivity of bacterial BlCel48B are significantly impacted by the cellulose size and its surface morphology. BlCel48B crystallographic structure was solved with ligands spanning -5 to -2 and +1 to +2 subsites. Statistical coupling analysis and molecular dynamics show that co-evolved residues on active site are critical for stabilizing ligands in the catalytic tunnel. Our results provide mechanistic insights into BlCel48B molecular-level determinants of activity, substrate binding, and processivity on insoluble cellulose, thus shedding light on structure-activity correlations of GH48 family members in general.
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