Journal
CARBOHYDRATE POLYMERS
Volume 262, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.117968
Keywords
Starch structure; ?-1; 6-glycosidic bond; Enzymatic modification; Molecular rearrangement; Transglycosylation efficiency
Categories
Funding
- National Natural Science Foundation of China [31722040, 31771935]
- National Firstclass Discipline Program of Food Science and Technology [JUFSTR20180204]
- Jiangsu province ?
- Collaborative Innovation Center of Food Safety and Quality Control?
- industry development program
Ask authors/readers for more resources
Enzymatically rearranging α-1,4 and α-1,6 glycosidic bonds in starch using a two-step modification process catalyzed by different GBE enzymes effectively reduces the digestibility of starch. The dual GBE modification process results in increased branching density, more abundant short branches, and shorter external chains in the modified starch, offering an efficient strategy for regulating starch digestibility.
Enzymatically rearranging ?-1,4 and ?-1,6 glycosidic bonds in starch is a green approach to regulating its digestibility. A two-step modification process successively catalyzed by 1,4-?-glucan branching enzymes (GBEs) from Rhodothermus obamensi STB05 (Ro-GBE) and Geobacillus thermoglucosidans STB02 (Gt-GBE) was investigated as a strategy to reduce the digestibility of corn starch. This dual GBE modification process caused a reduction of 25.8 % in rapidly digestible starch fraction in corn starch, which were more effective than single GBE-catalyzed modification with the same duration. Structural analysis indicated that the dual GBE modified product contained higher branching density, more abundant short branches, and shorter external chains than those in single GBEmodified product. These results demonstrated that a moderate Ro-GBE treatment prior to starch gelatinization caused several suitable alterations in starch molecules, which promoted the transglycosylation efficiency of the following Gt-GBE treatment. This dual GBE-catalyzed modification process offered an efficient strategy for regulating starch digestibility.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available