Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing

Composite dressing composed of Rhizochitosan and RegenplexTM to promote wound healing were assessed. Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6. Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration. In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue. The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process. Conclusively, the composite dressing demonstrated to be highly beneficial to the healing of fullthickness injury wound.

Automated summary

A composite dressing composed of Rhizochitosan and RegenplexTM was evaluated for promoting wound healing. The composite dressing showed better healing effects than individual components and control group, with possible mechanisms involving modulation of MMP-9 and MMP-2 expression levels to facilitate wound remodeling.