Long non-coding RNA DIO3OS binds to microRNA-130b to restore radiosensitivity in esophageal squamous cell carcinoma by upregulating PAX9

Esophageal squamous cell carcinoma (ESCC) ranks as one of the most deadly cancers worldwide due to its aggressive progression and poor treatment response. The long non-coding RNA (lncRNA)-microRNA (miRNA)-messenger RNA (mRNA) axis has been highlighted as a potency biomarker for enhancing the radiosensitivity of ESCC. Hence, we investigated the functional mechanism of the lncRNA DIO3OS/miR-130b/PAX9 axis in the radioresistance of ESCC cells. In cell experiments, we altered the miR-130b expression in ESCC cells using mimic or inhibitor to examine its effects on ESCC cell activities in response to 4 Gy irradiation, as well testing the involvement of lncRNA DIO3OS and the transcription factor gene PAX9. Tumor xenograft experiments were then conducted to observe the effect of miR-130b, lncRNA DIO3OS, and PAX9 on the radiosensitivity of ESCC cells in vivo. miR-130b was found to be highly expressed in ESCC. Downregulated miR-130b inhibited proliferation, invasion, and resistance to apoptosis in ESCC cells. LncRNA DIO3OS and PAX9 were downregulated in ESCC. The lncRNA DIO3OS could upregulate PAX9 by binding to miR-130b, which ultimately promoted the radiosensitivity of ESCC in vitro and in vivo. Taken together, lncRNA DIO3OS upregulates the expression of PAX9 by binding to miR-130b, ultimately promoting the radiosensitivity of ESCC.

Automated summary

The study found that lncRNA DIO3OS upregulates the expression of PAX9 by binding to miR-130b, ultimately promoting the radiosensitivity of esophageal squamous cell carcinoma (ESCC).