4.7 Article

WD40 repeat 43 mediates cell survival, proliferation, migration and invasion via vimentin in colorectal cancer

Journal

CANCER CELL INTERNATIONAL
Volume 21, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12935-021-02109-1

Keywords

WDR43; VIM; CRC; Biomarker

Categories

Funding

  1. National Nature Science Foundation of China [81670561]
  2. Natural Science Foundation of Jiangsu Province [QNRC2016022]
  3. Fundamental Research Funds for the Central Universities [021414380445]

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This study investigated the role of WDR43 in colorectal cancer, showing that its high expression is associated with increased tumorigenesis. Knockdown of WDR43 led to decreased proliferation, migration, invasion, and increased apoptosis in CRC cells. Additionally, the study found that WDR43 knockdown inhibited VIM expression and overexpression of VIM partially reversed the effects of WDR43.
Background WD40 repeat (WDR)43 is an RNA-binding protein that belongs to the WDR domain protein family. Its biological function is largely unclear, particularly in colorectal cancer (CRC). Methods In the present study, we searched the TCGA database and found the correlation between WDR43 and CRC. Subsequently, the high expression of WDR43 in human clinical samples of CRC was validated and we further examined the biological functions of it in CRC cells. Finally, we explored potential downstream proteins or pathways and established subcutaneous xenograft model to verify our findings. Results Immunohistochemistry of 16 patient specimens confirmed that the expression of WDR43 was elevated in CRC. WDR43 knockdown was shown to increase apoptosis and inhibit the proliferation, migration and invasion of CRC cells in vitro and reduce tumorigenesis in animal models. In addition, it was found that WDR43 knockdown inhibited vimentin (VIM) expression in CRC cells and overexpression of VIM can partially reverse the effects of WDR43 both in vitro and in vivo. Conclusion In conclusion, the role of WDR43 in the occurrence and development of CRC was investigated in the present study. WDR43 may serve as a valuable biomarker and provide new options for the diagnosis and treatment of colorectal cancer.

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