Journal
EXPERIMENTAL NEUROLOGY
Volume 283, Issue -, Pages 179-187Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.expneurol.2016.06.017
Keywords
Preterm infants; White matter brain injury; Oligodendrocytes; Umbilical cord blood therapy; Hypoxia-ischemia; Neuroprotection; Stem cell
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Funding
- NHMRC Australia [APP1081516]
- ARC [FT130100650]
- Inner Wheel Australia, L.E.W. Carty
- Victorian Government's Operational Infrastructure Support Program
- Australian Research Council [FT130100650] Funding Source: Australian Research Council
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Infants born very preterm are at high risk for neurological deficits including cerebral palsy. In this study we assessed the neuroprotective effects of umbilical cord blood cells (UCBCs) and optimal administration timing in a fetal sheep model of preterm brain injury. 50 million allogeneic UCBCs were intravenously administered to fetal sheep (0.7 gestation) at 12 h or 5 d after acute hypoxia-ischemia (HI) induced by umbilical cord occlusion. The fetal brains were collected at 10 d after HI. HI (n = 7) was associated with reduced number of oligodendrocytes (Olig2+) and myelin density (CNPase+), and increased density of activated microglia (lba-1+) in cerebral white matter compared to control fetuses (P < 0.05). UCBCs administered at 12 h, but not 5 d after HI, significantly protected white matter structures and suppressed cerebral inflammation. Activated microglial density showed a correlation with decreasing oligodendrocyte number (P < 0.001). HI caused cell death (TUNEL+) in the internal capsule and cell proliferation (Ki-67+) in the subventricular zone compared to control (P < 0.05), while UCBCs at 12 h or 5 d ameliorated these effects. Additionally, UCBCs at 12 h induced a significant systemic increase in interleukin-10 at 10 d, and reduced oxidative stress (malondialdehyde) following HI (P < 0.05). UCBC administration at 12 h after HI reduces preterm white matter injury, via anti-inflammatory and antioxidant actions. Crown Copyright (C) 2016 Published by Elsevier Inc All rights reserved.
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