Plasma glucagon-like peptide-1 responses to ingestion of protein with increasing doses of milk minerals rich in calcium

A high dose of whey protein hydrolysate fed with milk minerals rich in calcium (Capolac(R)) results in enhanced glucagon-like peptide-1 (GLP-1) concentrations in lean individuals; however, the effect of different calcium doses ingested alongside protein is unknown. The present study assessed the dose response of calcium fed alongside 25 g whey protein hydrolysate on GLP-1 concentrations in individuals with overweight/obesity. Eighteen adults (mean +/- sd: 8M/10F, 34 +/- 18 years, 28 center dot 2 +/- 2 center dot 9 kgm(-2)) completed four trials in a randomised, double-blind, crossover design. Participants consumed test solutions consisting of 25 g whey protein hydrolysate (CON), supplemented with 3179 mg (LOW), 6363 mg (MED) or 9547 mg (HIGH) Capolac(R) on different occasions, separated by at least 48 h. The calcium content of test solutions equated to 65, 892, 1719 and 2547 mg, respectively. Arterialised-venous blood was sampled over 180 min to determine plasma concentrations of GLP-1(TOTAL), GLP-1(7-36amide), insulin, glucose, NEFA, and serum concentrations of calcium and albumin. Ad libitum energy intake was measured at 180 min. Time-averaged incremental AUC (iAUC) for GLP-1(TOTAL) (pmol center dot l(-1)center dot min(-1)) did not differ between CON (23 +/- 4), LOW (25 +/- 6), MED (24 +/- 5) and HIGH (24 +/- 6). Energy intake (kcal) did not differ between CON (940 +/- 387), LOW (884 +/- 345), MED (920 +/- 334) and HIGH (973 +/- 390). Co-ingestion of whey protein hydrolysate with Capolac(R) does not potentiate GLP-1 release in comparison with whey protein hydrolysate alone. The study was registered at clinical trials (NCT03819972).

Automated summary

This study aimed to assess the impact of different calcium doses ingested alongside whey protein hydrolysate on GLP-1 concentrations in individuals with overweight/obesity. The results showed that co-ingestion of whey protein hydrolysate with Capolac(R) did not potentiate GLP-1 release compared to whey protein hydrolysate alone.