Genetic characterization of livestock-associated methicillin-resistant Staphylococcus aureus isolated in Greece

The interest in livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) strains is increasing due to their wide distribution and transmission even in persons without previous contact with livestock, and these strains pose a public health threat. The aim of the study was the genetic characterization of the whole genome of two epidemiologically unrelated t034 LA-MRSA strains previously isolated from the nasal cavities of a goat and a farmer in Greece. Both strains were assigned to the ST398-Vc-t034 type and they were carrying a single transposon identical to Tn6133. They harbored genes conferring resistance to several antibiotics (aminoglycosides, beta-lactams, macrolides, streptogramin B, tetracycline, and trimethoprim), and genes associated with virulence (enterotoxins, gamma-hemolysins, and aureolysin). The present study can serve as baseline for further LA-MRSA epidemiological and evolutionary studies in Greece, while awareness and increased surveillance are needed to avoid their spread.

Automated summary

The study genetically characterized two epidemiologically unrelated t034 LA-MRSA strains isolated from a goat and a farmer in Greece, showing that they carry genes conferring resistance to multiple antibiotics and genes associated with virulence. This research serves as a baseline for further LA-MRSA epidemiological and evolutionary studies in Greece, highlighting the need for increased awareness and surveillance to prevent their spread.