Journal
BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS
Volume 20, Issue 4, Pages 394-405Publisher
MS-EDITIONS
DOI: 10.37360/blacpma.21.20.4.29
Keywords
Anticancer; Apoptosis; Cellular antioxidant activity; Interleukin 6 and 8; Cervical cancer; Sideritis perfoliata
Funding
- Scientific Research Council of Gaziantep University [NMYO.YLT.17.01]
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The study showed that SPE reduced proliferation and induced apoptosis in HeLa cells, while also decreasing IL6-8 levels. The extract exhibited moderate antioxidant activities and contained high levels of vanillic acid, ultimately demonstrating anticancer properties.
In this study, it was aimed to determine the antioxidant and anticancer activities of Sideritis perfoliata methanolic extract (SPE) on cervical cancer cells (HeLa). Different doses (25, 50, 100 and 200 mu g/mL) of SPE were used to determine proliferation of HeLa cells by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) staining method. Induction of apoptosis was determined by Annexine-V and propidium iodide staining method. Interleukin (IL) 6-8 levels were measured by ELISA method. Antioxidant activities of SPE were determined by DPPH, DNA (plasmid pBR322) protecting and cellular antioxidant activity tests. Some phytochemicals of SPE were also screened by LC-MS-MS. It was determined that SPE reduced the proliferation of HeLa cells and also induced apoptosis. IL6-8 levels importantly decreased at 200 mu g/mL SPE exhibited moderately antioxidant activities in tests used. Among the phenolics identified, vanillic acid had the highest amount. As a result, it was determined to have the anticancer activity of SPE by decreasing cell proliferation, inducing apoptosis and decreasing IL6-8 in HeLa cells.
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