4.7 Article

Comparative analysis of latex transcriptomes reveals the potential mechanisms underlying rubber molecular weight variations between the Hevea brasiliensis clones RRIM600 and Reyan7-33-97

Journal

BMC PLANT BIOLOGY
Volume 21, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12870-021-03022-5

Keywords

Natural rubber; Hevea brasiliensis; Rubber molecular weight; Latex transcriptome; Rubber particle size

Categories

Funding

  1. National Natural Science Foundation of China [31872708]
  2. Central Public-interest Scientific Institution Basal Research Fund [1630022019019]
  3. National Key Research and Development Program of China [2019YFD1001102, 2019YFD1001105]

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The molecular weight and particle size of latex from different aged rubber trees vary, with tree age affecting the gene expressions related to rubber biosynthesis pathways and non-rubber isoprenoid pathways, ultimately influencing rubber quality. The study provides insights into the regulatory mechanisms of molecular weight in rubber biosynthesis and potential targets for genetic improvement of rubber quality.
Background: The processabilities and mechanical properties of natural rubber depend greatly on its molecular weight (MW) and molecular weight distribution (MWD). However, the mechanisms underlying the regulation of molecular weight during rubber biosynthesis remain unclear. Results: In the present study, we determined the MW and particle size of latex from 1-year-old virgin trees and 30-year-old regularly tapped trees of the Hevea clones Reyan7-33-97 and RRIM600. The results showed that both the MW and the particle size of latex varied between these two clones and increased with tree age. Latex from RRIM600 trees had a smaller average particle size than that from Reyan7-33-97 trees of the same age. In 1-year-old trees, the Reyan7-33-97 latex displayed a slightly higher MW than that of RRIM600, whereas in 30-year-old trees, the RRIM600 latex had a significantly higher MW than the Reyan7-33-97 latex. Comparative analysis of the transcriptome profiles indicated that the average rubber particle size is negatively correlated with the expression levels of rubber particle associated proteins, and that the high-MW traits of latex are closely correlated with the enhanced expression of isopentenyl pyrophosphate (IPP) monomer-generating pathway genes and downstream allylic diphosphate (APP) initiator-consuming non-rubber pathways. By bioinformatics analysis, we further identified a group of transcription factors that potentially regulate the biosynthesis of IPP. Conclusions: Altogether, our results revealed the potential regulatory mechanisms involving gene expression variations in IPP-generating pathways and the non-rubber isoprenoid pathways, which affect the ratios and contents of IPP and APP initiators, resulting in significant rubber MW variations among same-aged trees of the Hevea clones Reyan-7-33-97 and RRIM600. Our findings provide a better understanding of rubber biosynthesis and lay the foundation for genetic improvement of rubber quality in H. brasiliensis.

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