Chloroplast phylogenomics and divergence times of Lagerstroemia (Lythraceae)

Background: Crape myrtles, belonging to the genus Lagerstroemia L., have beautiful paniculate inflorescences and are cultivated as important ornamental tree species for landscaping and gardening. However, the phylogenetic relationships within Lagerstroemia have remained unresolved likely caused by limited sampling and the insufficient number of informative sites used in previous studies. Results: In this study, we sequenced 20 Lagerstroemia chloroplast genomes and combined with 15 existing chloroplast genomes from the genus to investigate the phylogenetic relationships and divergence times within Lagerstroemia. The phylogenetic results indicated that this genus is a monophyletic group containing four clades. Our dating analysis suggested that Lagerstroemia originated in the late Paleocene (similar to 60 Ma) and started to diversify in the middle Miocene. The diversification of most species occurred during the Pleistocene. Four variable loci, trnD-trnY-trnE, rrn16-trnI, ndhF-rpl32-trnL and ycf1, were discovered in the Lagerstroemia chloroplast genomes. Conclusions: The chloroplast genome information was successfully utilized for molecular characterization of diverse crape myrtle samples. Our results are valuable for the global genetic diversity assessment, conservation and utilization of Lagerstroemia.

Automated summary

The study sequenced 20 Lagerstroemia chloroplast genomes and combined them with 15 existing genomes to investigate phylogenetic relationships and divergence times. Results indicate Lagerstroemia is a monophyletic group with four clades, originating in the late Paleocene and diversifying in the middle Miocene. Most species diversified during the Pleistocene. Four variable loci were found in the chloroplast genomes.