4.8 Article

Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 (COVID-19)

Journal

BIOSENSORS & BIOELECTRONICS
Volume 185, Issue -, Pages -

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113177

Keywords

SARS-CoV-2; Electrochemical DNA detection; Point-of-care diagnostics; Capacitance transducer; Recyclable biosensor

Funding

  1. Ministry of Food and Drug Safety [21153MFDS431]
  2. Ajou University
  3. National Research Foundation (NRF) - Ministry of Science and ICT, Korea [NRF2020R1F1A1073564, 2018R1A6A1A03025242, 2018R1D1A1A09 083353]
  4. Korea Sports Promotion Foundation (KSPO) [DY0002258769-21153인프라431-1] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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This study introduces a label-free DNA capacitive biosensor for SARS-CoV-2 detection, offering real-time, low-cost, and high-throughput screening of nucleic acid samples. The biosensor validates specific DNA sequences' hybridization signals through comprehensive physicochemical analytical techniques, demonstrating significant sensitivity in detecting the conserved region of the SARS-CoV-2 RdRp gene.
Rapid diagnosis and case isolation are pivotal to controlling the current pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, a label-free DNA capacitive biosensor for the detection of SARS-CoV-2 that demonstrates real-time, low-cost, and high-throughput screening of nucleic acid samples is presented. Our novel biosensor composed of the interdigitated platinum/titanium electrodes on the glass substrate can detect the hybridization of analyte DNA with probe DNA. The hybridization signals of specific DNA sequences were verified through exhaustive physicochemical analytical techniques such as Fourier transform infrared (FT-IR) spectrometry, contact-angle analysis, and capacitance-frequency measurements. For a singlestep hybridized reaction, the fabricated kit exhibited significant sensitivity (capacitance change, Delta C = similar to 2 nF) in detecting the conserved region of the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) gene with high sensitivity of 0.843 nF/nM. In addition to capacitive measurements, this selective detection was confirmed by the fluorescence image and intensity from a SARS-CoV-2 gene labeled with a fluorescent dye. We also demonstrated that the kits are recyclable by surface ozone treatment using UV irradiation. Thus, these kits could potentially be applied to various types of label-free DNA, thereby acting as rapid, cost-effective biosensors for several diseases.

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