Journal
BIOSENSORS & BIOELECTRONICS
Volume 186, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113306
Keywords
3D-printing; Point-of-care molecular diagnostics; pH-sensitive electrode; Electrochemical detection; Loop-mediated; Isothermal amplification (LAMP)
Categories
Funding
- National Institutes of Health, United States [R01EB023607, R61AI154642, R01CA214072]
- Penn Center for AIDS Research, United States [P30 AI 045008]
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A simple, disposable, and integrated electronic-tube cap (E-tube cap) for DNA detection at the point-of-care was developed and successfully demonstrated the presence of 100 copies of λDNA in saliva samples. The device showed fast, accurate, and cost-effective diagnostic capabilities, making it particularly suitable for point-of-care molecular diagnostics of infectious diseases.
A simple, disposable, and integrated electronic-tube cap (E-tube cap) for DNA detection at the point-of-care was designed, fabricated, and tested. The E-tube cap contains a 3D printed electrode substrate for DNA extraction and label-free pH sensing detection. One Flinders Technology Associates (Whatman FTA) membrane was incorporated into the 3D printed electrode substrate for the isolation, concentration, and purification of DNA. The E-tube cap with captured DNA by the membrane was inserted directly into a reaction tube for loop-mediated isothermal amplification (LAMP). The isothermal amplification process was monitored in real-time by a 3D printed electrochemical electrode coated with pH-sensitive material (carbon/iridium oxide layer). The pH sensing electrode showed an excellent linear response within the pH range of 6-9 with a slope of -31.32 +/- 0.5 mV/pH at room temperature. The utility of the integrated E-tube cap was demonstrated by detecting the presence of lambda DNA spiked in saliva samples with a sensitivity of 100 copies per mL sample within 30 min. Such a simple, rapid, and affordable diagnostic device is particularly suitable for point-of-care molecular diagnostics of infectious diseases.
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