Journal
BIOSENSORS & BIOELECTRONICS
Volume 184, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113219
Keywords
Fluorescence correlation spectroscopy; Plasmonics; Super-localization; Plasmonic nanodimer arrays; Nanogap; Lysosome
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Funding
- National Research Foundation (NRF) - Korean Government [2019R1A4A1025958]
- Korea Medical Device Development Fund [202011D25]
- NRF [2019R1A6A1A03032869]
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The report focuses on plasmon-enhanced imaging fluorescence correlation spectroscopy, utilizing nanogap-based plasmonic nanodimer arrays for improved signal-to-noise ratio and precision. The study confirms the enhancement effect of PNAs and investigates lysosomes diffusion dynamics, potentially serving as a powerful analytical tool for exploring subcellular dynamics in the future.
In this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.
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