Journal
BIOSENSORS & BIOELECTRONICS
Volume 181, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113123
Keywords
Electrochemiluminescence; Oxygen consumption; Mesenchymal stem cell spheroid; Luminol; Live-cell imaging
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Funding
- Japan Society for the Promotion of Science [20H00619, 18H01840, 18H01999, 19K20658, 20J21401]
- Program for Creation of Interdisciplinary Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University
- Shimadzu Science Foundation
- Nakatani Foundation
- Kato Foundation for Promotion of Science
- Murata Science Foundation
- JST COI [JPMJCE1303]
- Grants-in-Aid for Scientific Research [19K20658, 20H00619, 18H01999, 20J21401, 18H01840] Funding Source: KAKEN
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This study demonstrates the electrochemiluminescence (ECL) imaging of spheroid respiratory activity for the first time, providing visualization of O2 distribution and enabling time-lapse imaging for studying cellular dynamics in spheroids with promising high-throughput imaging strategy.
The respiratory activity of cultured cells can be electrochemically monitored using scanning electrochemical microscopy (SECM) with high spatial resolution. However, in SECM, the electrode takes a long time to scan, limiting simultaneous measurements with large biological samples such as cell spheroids. Therefore, for rapid electrochemical imaging, a novel strategy is needed. Herein, we report electrochemiluminescence (ECL) imaging of spheroid respiratory activity for the first time using sequential potential steps. L-012, a luminol analog, was used as an ECL luminophore, and H2O2, a sensitizer for ECL of L-012, was generated by the electrochemical reduction of dissolved O2. The ECL imaging visualized spheroid respiratory activity?evidenced by ECL suppression?corresponding to O2 distribution around the spheroids. This method enabled the time-lapse imaging of respiratory activity in multiple spheroids with good spatial resolution comparable to that of SECM. Our work provides a promising high-throughput imaging strategy for elucidating spheroid cellular dynamics.
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