Journal
BIORESOURCE TECHNOLOGY
Volume 336, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125323
Keywords
Syngas-derived acetate; (R)-3-hydroxybutyric acid; propionyl-CoA transferase; Clostridium beijerinckii; Escherichia coli
Funding
- Fok YingTong Education Foundation, China [161017]
- National Natural Science Foundation of China [21776083]
- Natural Science Foundation of Shanghai [19ZR1472700]
- Fundamental Research Funds for the Central Universities [22221818014]
- 111 Project [B18022]
- CAS Key Laboratory of Synthetic Biology
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Acetate is a potential non-food carbon source for industrial production to address the shortage of food-based feedstocks. This study successfully constructed a biosynthesis pathway for R-3HB using engineered E. coli strains, which efficiently utilized syngas-derived acetate to synthesize R-3HB.
Acetate is a potential non-food carbon source for industrial production, coping with the shortage of food-based feedstocks. (R)-3-hydroxybutyric acid (R-3HB) can be used as an important chiral intermediate in the fine chemical and pharmaceutical industry. In this study, the R-3HB biosynthesis pathway was successfully constructed when genes of beta-ketothiolase (phaA), acetoacetyl-CoA reductase (phaB) from Ralstonia eutropha, and propionyl-CoA transferases (pct) from Clostridium beijerinckii 8052 were introduced into Escherichia coli. The effects of host E. coli strains, different propionyl-CoA transferases, and post-induction temperatures were investigated. The final concentration of R-3HB reached 0.86 g/L using acetate as the sole carbon source. Subsequently, a kind of culture broth containing the syngas-derived acetate was used to produce 1.02 g/L of R-3HB with a yield of 0.26 g/g. In this study, the engineered E. coli strain could efficiently utilize syngas-derived acetate to synthesize R-3HB.
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