Journal
BIORESOURCE TECHNOLOGY
Volume 330, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.124981
Keywords
Syringyl lignin; Hardwood; Proteomics; Genomics; Malic acid
Funding
- UGC Government of India
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This study demonstrates the efficient utilization of syringaldehyde and syringic acid by Burkholderia sp. ISTR5, with proteins such as aldehyde dehydrogenase, laccase, and oxidoreductases being upregulated during the degradation process. R5 was able to completely transform both substrates within a short time frame and produce a significant amount of malic acid, showcasing its potential for industrial applications in biorefinery processes.
Syringyl monomeric units are the most common intermediates encountered during hardwood lignin degradation. In the present study, efficient utilization of syringaldehyde (SAld), syringic acid (SAc) by Burkholderia sp. ISTR5 (R5) has been shown. The proteogenomic analysis of Burkholderia sp. ISTR5 was done to understand the enzymes involved in the degradation of syringaldehyde and syringic acid. Various proteins such as aldehyde dehydrogenase, laccase, and oxidoreductases were highly upregulated during growth on syringaldehyde and syringic acid. R5 completely transformed both the substrates SAld and SAc to other hydrocarbons in 48 h and 24 h, respectively. Moreover, bioconversion of syringyl lignins followed an unusual pathway and accumulated a considerable amount of industrially valuable chemical malic acid in the reaction titer. This study shows the robust chassis of R5 to cope with the aromatic aldehydic stress and simultaneous bioconversion into valuable products for an efficient biorefinery.
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