Journal
BIORESOURCE TECHNOLOGY
Volume 342, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125979
Keywords
Corncob; Acetic acid/sodium acetate hydrolysis; Xylanase; Xylooligosaccharides; Monosaccharides
Funding
- Nanjing Forestry University [163030103]
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This study optimized the production of xylooligosaccharides (XOS) and monosaccharides from corncobs using acid-base conjugate system hydrolysis and enzyme hydrolysis, achieving high yields without the need for xylan isolation and purification.
At present, xylooligosaccharides (XOS) from corncob using acid-base conjugate system has not been reported. In this study, XOS production from corncob by two-step acetic acid/sodium acetate (AC/SA) conjugate system hydrolysis and xylanase hydrolysis was optimized, and monosaccharides were subsequently produced from corncob residues by cellulase hydrolysis. The XOS of 19.9 g/L was obtained from corncob (10%, w/v) using 0.15 M AC/SA hydrolysis at a molar ratio of 3.0 at 170 degrees C for 60 min, followed by xylanase hydrolysis. The second step AC/SA hydrolysis of hydrolyzed corncob (10%, w/v) produced 3.1 g/L of XOS. Finally, the maximum XOS yield of 74.8% (based on xylan in corncob) was achieved, which is the highest yield among yields reported previously. The purity of XOS was high, whereas the contents of by-products were very low. This work presents a novel and promising strategy for co-production of XOS and monosaccharides from corncob without xylan isolation and purification.
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