Lower C/N ratio induces prior utilization of soluble microbial products with more dramatic variability and higher biodegradability in denitrification by strain YSF15

The emphasis of this study lies in how strain SYF15 regulates molecular weight (MW) fractions of soluble microbial products (SMPs) in response to low carbon to nitrogen (C/N) ratio, with high denitrification performance (over 99%). Results indicated SMPs with MW 100 and <50 kDa undoubtedly participated in denitrification before 12.0 h in C/N = 2.0, while sodium acetate was preferred in C/N = 5.0, indicating strain YSF15 was induced to degrade SMPs as a carbon source in low C/N. Additionally, lower C/N activated the extracellular metabolism, with increased fluorescence regional integration (FRI) volume amplitude by 48.08 and 53.43% (versus C/N = 5.0) in MW = 50-10 and 10-3 kDa, respectively. The FRI volume of proteins yielded greater with more degradable components than higher C/N in MW = 100-3 kDa, whereas polysaccharide and protein concentrations differed little with considerable biodegradability, implying components inside protein changed dramatically. This pioneering work contributed to the understanding of denitrification with carbon source deficiency.

Automated summary

The study focuses on how strain SYF15 regulates the molecular weight fractions of soluble microbial products in response to low carbon to nitrogen ratio, with high denitrification performance. Results show that specific molecular weight SMPs are involved in denitrification and that low C/N activates extracellular metabolism, increasing fluorescence regional integration volume in specific molecular weight ranges.