Journal
BIORESOURCE TECHNOLOGY
Volume 333, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125075
Keywords
Biorefinery; Downstream processing; Liquid triphasic flotation; Microalgae
Funding
- Fundamental Research Grant Scheme, Malaysia [FRGS/1/2019/STG05/UNIM/02/2]
- MyPAIR-PHC-Hibiscus Grant [MyPAIR/1/2020/STG05/UNIM/1]
- Ministry of Science and Technology, Taiwan [MOST 109-3116-F-006-016-CC1, 109-2621-M-029-001, 107-2221-E-006-112-MY3]
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This study aims to extract multiple components using a single processing unit, incorporating an ultrasound-assisted liquid triphasic flotation system to extract proteins, lipids, and carbohydrates through phase separation. The biorefinery approach is crucial for commercializing microalgae for biodiesel and bioethanol production.
Microalgae are potential sustainable renewable sources of energy but are highly underutilized due to the expensive and time-consuming downstream processing. This study aims at curbing these obstacles by extracting multiple components with a single processing unit. In this work, an ultrasound-assisted liquid triphasic flotation system was incorporated to extract proteins, lipids, and carbohydrates by phase separation. The parameters involved were optimized and the final recovery efficiency of proteins, lipids, and carbohydrates was determined. A control run involving conventional three-phase partitioning and a 15-fold scale-up system with the recycling of phase components were also performed. Gas Chromatograph and Fourier Transform Infrared spectroscopy were used to examine the potential of extracted products as a source of biofuel. This biorefinery approach is crucial in commercializing microalgae for biodiesel and bioethanol generation with a side product of purified proteins as feed.
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