Journal
BIORESOURCE TECHNOLOGY
Volume 329, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.124929
Keywords
Microwave pretreatment; Clostridium be?erinck?; Sugarcane processing; Box-Behnken design
Funding
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2018/00789-7]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil [CAPES 001]
- Regional Government of Castilla y Leon
- EU-FEDER [VA010P17, CLU 2017-09, UIC 129]
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Sugarcane straw was pretreated with dilute sulfuric acid assisted by microwave to increase fermentable sugars and reduce inhibitors in the hydrolysates. Optimized conditions allowed for enzymatic hydrolysis and fermentation without detoxification, making bio-butyric acid production from SCS highly attractive.
Sugarcane straw (SCS) was pretreated with dilute sulfuric acid assisted by microwave to magnify fermentable sugars and to minimize the concentration of inhibitors in the hydrolysates. The optimum conditions for maximum recovery of sugars were 162 ?C and 0.6% (w/v) H2SO4. The low level of inhibitors, such as acetate (2.9 g/L) and total phenolics (1.4 g/L), in the SCS slurry from the pretreatment stage allowed the enzymatic hydrolysis and fermentation steps to occur without detoxification. Besides consuming the total sugar content (31.0 g/L), Clostridium beijerinckii Br21 was able to use acetate from the SCS hydrolysate, to give butyric acid at high conversion factor (0.49 g of butyric acid /g of sugar). The optimized pretreatment conditions spared acid, time, and the detoxification stage, making bio-butyric acid production from SCS extremely attractive.
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