Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 44, Issue 11, Pages 2387-2398Publisher
SPRINGER
DOI: 10.1007/s00449-021-02611-w
Keywords
Cordycepin; Co-60-gamma ray irradiation; Paecilomyces hepiali; Medium screening; Response surface methodology
Funding
- National Natural Science Foundation of China [32070099, 32000898]
- Natural Science Foundation of Zhejiang Province [LQ21B060006]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [RF-C2019005]
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The study successfully increased the yield of cordycepin through strain modification and medium screening, resulting in a high-yield mutant strain P. hepiali ZJB18001, which offers new possibilities for the industrial production of cordycepin.
Cordycepin is a major bioactive compound found in Cordyceps sinensis that exhibits a broad spectrum of biological activities. Here a Paecilomyces hepiali OR-1 strain was initially isolated from plateau soil for the bioproduction of cordycepin. Subsequently, strain modification including Co-60 gamma-ray and ultraviolet irradiation were employed to increase the cordycepin titer, resulted in a high-yield mutant strain P. hepiali ZJB18001 with the cordycepin content of 0.61 mg/g(DCW), showing a 2.3-fold to that from the wild strain (0.26 mg/g(DCW)). Furthermore, medium screening based on Box-Behnken design and the response surface methodology facilitated the enhancement of cordycepin yield to the value of 0.96 mg/g(DCW) at 25 degrees C for 5 days in submerged cultivation with an optimized medium composition. The high cordycepin yield, rapid growth rate and stable genetic characteristics of P. hepiali ZJB18001 are beneficial in terms of costs and time for the industrialization of cordycepin production.
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