UV-fluorescence probe for detection Ni2+with colorimetric/spectral dual-mode analysis method and its practical application

Fluorescence probe combines with fluorescence imaging technology has become the most powerful analytical method with their great advantages of high sensitivity and selectivity and real-time monitoring. Ni2+ is widely distributed in food, environment and living animals, thereof, it is of great significance for detection Ni2+ with high selectivity. Herein, a simple strategy is proposed to design and synthesiz a small molecule fluorescent probe Y1 by using one-pot method. The spectroscopic behaviors including UV-Vis absorption and fluorescence emission spectrum have been used to verify the feasibility of probe towards Ni2+ in water/EtOH (v/v = 2:8) mixtures under neutral condition. As expected, Y1 offers high selectivity and sensitivity for detection Ni2+ in aqueous solution with a good linear relationship and low detection limit within Ni2+ concentration variation from 0 to 13 mu M (DOL = 0.0038 mu M, R-2 = 0.9983). It is remarkable that Y1 can be applied for real-time visualization Ni2+ change in sprouted potato and zebrafish with great photo-stability, highlighting that the practicability and feasibility of Y1 to detect and monitor Ni2+ in the field of food industry and biomedical field.

Automated summary

The fluorescence probe Y1 shows high selectivity and sensitivity for detecting Ni2+ in aqueous solution, with real-time visualization capability and a good linear relationship within a certain concentration range. It can be applied for monitoring Ni2+ changes in sprouted potato and zebrafish with great photo-stability, demonstrating practicality and feasibility in the fields of food industry and biomedical research.