4.7 Review

Therapeutic and diagnostic relevance of Crispr technology

Journal

BIOMEDICINE & PHARMACOTHERAPY
Volume 138, Issue -, Pages -

Publisher

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2021.111487

Keywords

Crispr; Cas protein; Genome editing; Bacteria

Ask authors/readers for more resources

CRISPR, a family of DNA repeats, provides immunity against viral and plasmid DNA in bacteria and archaea by cleaving DNA double strands at specific sites using endonuclease Cas guided by RNA sequence. The discovery revolutionized genetic engineering, allowing for precise genome editing. While CRISPR/Cas system has vast applications in biomedical field, ethical concerns may hinder its full potential.
CRISPR is a family of DNA repeats providing immunity against viral and plasmid invading DNA in bacteria and archaea. The system consist of an endonuclease Cas, guided by a RNA sequence, able to cleave the DNA double strand at a specific site. The discovery of Crispr function in 2007 has revolutionized genetic engineering by giving to the world the most powerful and precise tool for targeted genome editing. The aim of this review is to synthesize the current knowledge on Crispr/cas system and its application in biomedical field. In particular, we focus on the relevance of this new tool in progressing our comprehension for biological mechanisms and improving our ability to treat and prevent genetic diseases, to control microbial virulence and to generate animal models for basic and clinical research. We discuss also the ethical issues that may prevent the application of Crispr technology in living beings.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available