Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 35, Issue 11, Pages -Publisher
WILEY
DOI: 10.1002/bmc.5182
Keywords
phenolic acids; pH-zone-refining counter-current chromatography; preparative separation; Salvia yunnanensis Radix
Categories
Funding
- Earmarked Fund for China Agriculture Research System [CARS-21]
- National Natural Science Foundation of China [21904080]
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Phenolic acids are the main active components in Salvia yunnanensis Radix, which can be effectively separated using pH-zone-refining counter-current chromatography (pH-ZRCCC). This study successfully separated phenolic acids from S. yunnanensis Radix and provided technical support for quality control research.
Phenolic acids are the main active components in Salvia yunnanensis Radix, which have significant effects such as cardiovascular protection, anti-thrombosis, anti-oxidant, and anti-inflammation. In this study, pH-zone-refining counter-current chromatography (pH-ZRCCC) was successfully applied to the preparative separation of phenolic acids from S. yunnanensis Radix. First, a two-phase solvent system composed of Pet-EtOAc-ACN-H2O (1.5:2.5:1:5, v/v) [TFA (10 mM) was added in the upper phase and NH3 center dot H2O (30 mM) was added in the lower phase] was used for the separation of 4.0 g of the crude sample to obtain 55.6 mg of rosmarinic acid (1), 69.0 mg of caffeic acid (2), 18.9 mg of protocatechualdehyde (3), 14.6 mg of 8-epiblechnic acid 9-methyl ester (4), and a mixture containing two compounds. After the recovery, 1.3 g of the mixture was obtained and separated using the MtBE-H2O (1:1, v/v) solvent system containing TFA (5 mM) and NH3 center dot H2O (60 mM) to obtain 259.9 mg of salvianolic acid B (5) and 28.75 mg of lithospermic acid (6). Moreover, a systematic separation pattern for separating the relatively low-polarity phenolic acids from natural products by pH-ZRCCC was summarized for the first time. This study provided technical support for the pharmacological activity and quality control research of S. yunnanensis Radix.
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