Journal
BIOFOULING
Volume 37, Issue 6, Pages 656-665Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2021.1954169
Keywords
Photodynamic therapy; chlorin e6; hydrogen peroxide; biofilm; oxygen
Funding
- Research and Development Foundation of the West China Hospital of Stomatology, Sichuan University [RD-02-201905]
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This study demonstrates that the combination of 33.3 mM H2O2 and Ce6-aPDT significantly enhances antimicrobial efficacy, particularly for biofilm infections thriving in anaerobic microenvironments.
Antimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 mu M), H2O2 (0.3, 3.3, 33.3 mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm(2))(-1) (lambda = 450 nm or 660 nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3 mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment.
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