Journal
BIOESSAYS
Volume 43, Issue 8, Pages -Publisher
WILEY
DOI: 10.1002/bies.202100052
Keywords
enzyme engineering; genetic diversity; library creation methods; sequence space exploration; smart library design
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada [PVX20965-(RGP)]
Ask authors/readers for more resources
Enzyme engineering allows for exploring sequence diversity to find new properties. The literature is full of methods for creating enzyme libraries, and this article aims to help both novices and experts select the best strategies for their purposes.
Enzyme engineering allows to explore sequence diversity in search for new properties. The scientific literature is populated with methods to create enzyme libraries for engineering purposes, however, choosing a suitable method for the creation of mutant libraries can be daunting, in particular for the novices. Here, we address both novices and experts: how can one enter the arena of enzyme library design and what guidelines can advanced users apply to select strategies best suited to their purpose? Section I is dedicated to the novices and presents an overview of established and standard methods for library creation, as well as available commercial solutions. The expert will discover an up-to-date tool to freshen up their repertoire (Section I) and learn of the newest methods that are likely to become a mainstay (Section II). We focus primarily on in vitro methods, presenting the advantages of each method. Our ultimate aim is to offer a selection of methods/strategies that we believe to be most useful to the enzyme engineer, whether a first-timer or a seasoned user.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available