4.7 Article

Glucose-Sensitive Core-Cross-Linked Nanoparticles Constructed with Polyphosphoester Diblock Copolymer for Controlling Insulin Delivery

Journal

BIOCONJUGATE CHEMISTRY
Volume 32, Issue 9, Pages 2095-2107

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.1c00390

Keywords

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Funding

  1. National Natural Science Foundation of China [21975169, 21374066]
  2. Young Talent Program of China National Nuclear Corp. [CNNC51007]
  3. Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions

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This study successfully constructed biocompatible, biodegradable nanoparticles sensitive to glucose for insulin release in high blood glucose environments. The nanoparticles showed promising potential for diabetes treatment through controlled insulin release.
This work aims to construct biocompatible, biodegradable core-cross-linked and insulin-loaded nanoparticles which are sensitive to glucose and release insulin via cleavage of the nanoparticles in a high-concentration blood glucose environment. First, a polyphosphoester-based diblock copolymer (PBYP-g-Gluc)-b-PEEP was prepared via ring-opening copolymerization (ROP) and the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) in which PBYP and PEEP represent the polymer segments from 2-(but-3-yn-1-yloxy)-2-oxo-1,3,2-dioxaphospholane and 2-ethoxy-2-oxo-1,3,2-dioxaphospholane, respectively, and Gluc comes from 2-azidoethyl-beta-D-glucopyranoside (Gluc-N-3) that grafted with PBYP. The structure and molecular weight of the copolymer were characterized by H-1 NMR, P-31 NMR, GPC, FT-IR, and UV-vis measurements. The amphiphilic copolymer could self-assemble into core-shell uncore-cross-linked nanoparticles (UCCL NPs) in aqueous solutions and form core-cross-linked nanoparticles (CCL NPs) after adding cross-linking agent adipoylamidophenyl boronic acid (AAPBA). Dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to study the self-assembly behavior of the two kinds of NPs and the effect of different Gluc group contents on the size of NPs further to verify the stability and glucose sensitivity of CCL NPs. The ability of NPs to load fluorescein isothiocyanate-labeled insulin (FITC-insulin) and their glucose-triggered release behavior were detected by a fluorescence spectrophotometer. The results of methyl thiazolyl tetrazolium (MTT) assay and hemolysis activity experiments showed that the CCL NPs had good biocompatibility. An in vivo hypoglycemic study has shown that FITC-insulin-loaded CCL NPs could reduce blood glucose and have a protective effect on hypoglycemia. This research provides a new method for constructing biodegradable and glucose-sensitive core-cross-linked nanomedicine carriers for controlled insulin release.

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