Journal
BIOCHEMISTRY-MOSCOW
Volume 86, Issue 7, Pages 887-897Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297921070099
Keywords
isothermal amplification; background synthesis; DNA polymerases; LAMP; SDA; EXPAR
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The review discusses the main factors and approaches to prevent the formation of nonspecific products in isothermal nucleic acid amplification.
The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR.
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