Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 171, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bej.2021.108004
Keywords
Enzymatic carboxylation; CO2; Isocitrate dehydrogenase; Glucose dehydrogenase; Cofactor regeneration
Funding
- JSPS KAKENHI [JP19K05560]
- Kobayashi Foundation
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The study achieved carboxylation through enzymatic reaction under specific conditions, resulting in increased yield.
Thermodynamically-constrained carboxylation reaction requires high temperature and pressure in industrial processes. Research endeavors are aimed towards developing safer and greener alternatives. Enzymatic systems are considered as potential substitutes. In this study, we conducted direct carboxylation via a bi-enzymatic reaction with Thermoplasma acidophilum isocitrate dehydrogenase (TaIDH) and T. acidophilum glucose dehydrogenase (TaGDH) to produce isocitric acid from of 2-ketoglutaric acid under supercritical CO2. Coupling with the TaGDH reaction (for cofactor regeneration) enabled the equilibrium of the reactions to shift towards carboxylation, resulting in a 16-fold increase in yield. Furthermore, the yield was increased by optimizing the reaction parameters such as pH, substrate concentration, temperature, and time, and by testing various metal ions. A preparative scale reaction was conducted successfully.
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