4.6 Article

Synthesis and characterization of cross-linked lipase-metal hybrid nanoflowers on graphene oxide with increasing the enzymatic stability and reusability

Journal

BIOCHEMICAL ENGINEERING JOURNAL
Volume 172, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.bej.2021.108038

Keywords

Immobilization; Nanobiocatalyst; Lipase; Activity; Stability; Reusability

Funding

  1. Research Council of the Shahid Bahonar University of Kerman, Kerman (Iran)

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In the study, a purified lipase from Enterobacter sp. MG10 was immobilized using different methods, with CGLNF showing broader activity, higher efficiency, significantly reduced K-m value towards pNPP substrate, and high efficiency in converting prosopis stephanina oil to biodiesel. The results indicate the high potential of CGLNF nanobiocatalyst in synthetic biotechnology, particularly in biodiesel production.
In the current study, a purified lipase from Enterobacter sp. MG10 has been immobilized in three approaches, including lipase nano-flower (LNF), LNF/graphene oxide (GLNF), and cross-linked GLNF (CGLNF). The encapsulation yield (EY) and activity recovery (AR) were obtained in the range of 40.5 % to 81.0 % and 71.5 % to 132.2 % for lipase MG10, respectively. The best activity recovery was found (132.2 % for 0.02 mg ml(-1) enzyme) in the presence of 200 mM of Cu2+ for 72 h incubation at 4 degrees C. The CGLNF showed broader pH and temperature activities compared to free lipase. Moreover, the CGLNF displayed a 60 % improvement in lipase activity after 28 days of incubation, compared to free lipase. Remarkably, the K-m value of CGLNF was 0.980 mM toward the pNPP substrate, whereas the K-m value of free lipase was 17.11 mM at the same condition. Furthermore, the catalytic efficiency of CGLNF was 430.45 S-1 mM(-1), compared with 21.32 S-1 mM(-1) for the free lipase. The CGLNF showed 93 % efficiency in converting prosopis stephanina oil to biodiesel, whereas it found 63 % for the free lipase. Overall, these results showed the high potential of CGLNF nanobiocatalyst in synthetic biotechnology, especially in biodiesel production.

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