A novel C-type lectin from Crassostrea gigas involved in the innate defense against Vibrio alginolyticus

C-type lectins (CTLs) are important immune molecules that participate in invertebrate defense response. In the present work, a novel structural CTL (CgLec-4E) was identified from Crassostrea gigas, which encodes 237 amino acids (aa) with an extra long chain of aa and in the C-type CRD domain with EPA, QPG and WHD mutated motifs respectively. rCgLec-4E could agglutinate and inhibit the growth of Vibrio alginolyticus, except Chlorella, which might be relevant to three mutated motifs. CgLec-4E was mainly expressed in digestive gland, and its expression level was significantly up-regulated post V. alginolyticus challenge, indicating that the high expression of CgLec-4E could provide necessary mucosal immune protections and might involve in food particle recognition for C. gigas. Moreover, the subcellular locations indicated that CgLec-4E might play different roles in the immune response. Taken together, our results enrich our understanding of the structures and function of CTLs in invertebrates. (c) 2021 Elsevier Inc. All rights reserved.

Automated summary

This study identified a novel structural CTL (CgLec-4E) in Crassostrea gigas, which has antimicrobial and immune protective functions, and may be involved in food particle recognition. The results enrich understanding of the structures and functions of CTLs in invertebrates.