Journal
EXPERIMENTAL CELL RESEARCH
Volume 343, Issue 2, Pages 126-134Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2016.03.012
Keywords
Colon cancer; Aryl Hydrocarbon receptor; Gut immunity; Tumor suppression
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Funding
- Ministry of Education, Culture, Sports, and Technology in Japan [23114509, 22501001, 26430131]
- Grants-in-Aid for Scientific Research [26430131, 22501001] Funding Source: KAKEN
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Intestinal homeostasis is maintained by complex interactions between intestinal microorganisms and the gut immune system. Dysregulation of gut immunity may lead to inflammatory disorders and tumorigenesis. We previously have shown the tumor suppressive effects of aryl hydrocarbon receptor (AhR) in intestinal carcinogenesis. In the present study, we investigated AhR distribution in the mouse and human intestine by histochemical analysis. In the normal intestine, AhR was mainly localized in the stroma containing immune cells in the lamina propria and lymphoid follicles. On the other hand, in the tumor tissue from human colon cancer and that developed in Apc(Min/+) mice, AhR expression was elevated. AhR immunostaining was found in both stromal and tumor cells. Although AhR was localized in the cytoplasm of tumor cells in most cases, nuclear AhR was also observed in some. AhR knockdown using siRNA resulted in significant promotion of cell growth in colon cancer cell lines. Furthermore, AhR activation by AhR ligands supplemented in culture medium suppressed cell growth. Our study results suggest that tumor suppressive roles of AhR are estimated in two distinct ways: in normal tissue, AhR is associated with tumor prevention by regulating gut immunity, whereas in tumor cells, it is involved in growth suppression. (c) 2016 Elsevier Inc. All rights reserved.
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