Journal
BEHAVIOURAL BRAIN RESEARCH
Volume 412, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbr.2021.113430
Keywords
Autism; Pup mouse model; Vocal phenotype; Body size; Ultrasonic calls; Clicks
Categories
Funding
- Russian Science Foundation [19-14-00037]
- Russian Foundation for Basic Research [16-04-00534]
- Unique scientific installation Biological collection - Genetic biomodels of neuro-psychiatric disorders of the autism spectrum disorders of the Federal State Budgetary Scientific Institution Scientific Research Institute of Neurosciences and Medicine [493387, AAAA-A16-116021010228-0]
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This study identified a new acoustic phenotype in Calsyntenin2 deficient mice during the neurodevelopment period, showing differences in ultrasonic calls and increased head-to-body ratio. Lack of calsyntenin2 affected vocalization in mice, with higher call rates in certain discomfort conditions. The emission of wideband ultrasonic calls remained consistent as a reliable indicator in Clstn2-KO mice.
This study discovered a novel acoustic phenotype in Calsyntenin2 deficient knockout (Clstn2-KO) pups in the neurodevelopment period of 5-9 postnatal days (PND 5-9). The narrowband ultrasonic calls (nUSVs) were less complex (mostly one-note, shorter in duration and higher in peak frequency) in Clsnt2-KO than in wild-type (WT) C57BL/6 J pups. The wideband ultrasonic calls (wUSVs) were produced substantially more often by Clstn2-KO than WT pups. The clicks were longer in duration and higher in peak frequency and power quartiles in Clstn2-KO pups. The elevated discomfort due to additional two-minute maternal separation coupled with experimenter's touch, resulted in significantly higher call rates of both nUSVs and clicks in pups of both genotypes and sexes compared to the previous two-minute maternal separation, whereas the call rate of wUSVs was not affected. In Clstn2-KO pups, the prevalence of emission of wUSVs retained at both sex and both degrees of discomfort, thus providing a reliable quantitative acoustic indicator for this genetic line. Besides the acoustic differences, we also detected the increased head-to-body ratio in Clstn2-KO pups. Altogether, this study demonstrated that lack of such synaptic adhesion protein as calsyntenin2 affects neurodevelopment of vocalization in a mouse as a model organism.
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