Journal
EXPERIMENTAL CELL RESEARCH
Volume 340, Issue 1, Pages 150-158Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2015.12.009
Keywords
Mesenchymal stem cell; Extracellular vesicles; Paracrine effect; ALS; Motoneuron disease; Motoneuron-like NSC-34
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Funding
- AXonomIX research project - Provincia Autonoma di Trento, Italy [000040102362 PAT]
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Therapeutic strategies for the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS) have not yet provided satisfactory results. Interest in stem cells for the treatment of neurodegenerative diseases is increasing and their beneficial action seems to be due to a paracrine effect via the release of exosomes, main mediators of cell cell communication. Here we wished to assess, in vitro, the efficacy of a novel non-cell therapeutic approach based on the use of exosomes derived from murine adipose-derived stromal cells on motoneuron-like NSC-34 cells expressing ALS mutations, and used as in vitro models of disease. In particular, we set out to investigate the effect of exosomes on NSC-34 na ve cells and NSC-34 cells overexpressing human SOD1(G93A) or SOD1(G37R) or SOD1(A4V) mutants, exposed to oxidative stress. The data presented here indicate for the first time that exosomes (0.2 mu g/ml) are able to protect NSC-34 cells from oxidative damage, which is one of the main mechanism of damage in ALS, increasing cell viability. These data highlight a promising role of exosomes derived from stem cells for potential therapeutic applications in motoneuron disease. (C) 2015 Elsevier Inc. All rights reserved.
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