Journal
AUTISM RESEARCH
Volume 14, Issue 9, Pages 1837-1842Publisher
WILEY
DOI: 10.1002/aur.2568
Keywords
autism spectrum disorder; diffusion tensor imaging; magnetic resonance imaging; Shank3
Categories
Funding
- Autism Speaks
- Beatrice and Samuel A. Seaver Foundation
- National Institute of Mental Health [5R01 MH101584, F31 MH115656]
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Mutations and deletions in the SHANK3 gene cause Phelan-McDermid syndrome, characterized by intellectual disability and autism spectrum disorder. Studies on both patients and rat models with Shank3 deficiency show reductions in brain volume and white matter alterations.
Mutations and deletions in the SHANK3 gene cause the major neurodevelopmental features of Phelan-McDermid syndrome (PMS), which is characterized by intellectual disability, autism spectrum disorder, and sensory hyporeactivity. SHANK3 encodes a key structural component of excitatory synapses important for synaptogenesis. Clinical assessments and limited brain imaging studies of patients with PMS have uncovered regional volume reductions and white matter thinning. While these impairments have been replicated ex vivo in pups of a rat model, brain structure has not been assessed in rats in vivo or in adults. We assessed the brain structure of heterozygous and homozygous adult Shank3-deficient male rats in comparison to wild-type littermates with magnetic resonance imaging using both anatomical assessments and diffusion tensor imaging (DTI). Shank3-deficient rats showed a reduction in overall brain size and the absolute volume of the neocortex, piriform cortex, thalamus, forebrain, inferior and superior colliculi, internal capsule, and anterior commissure. The superior colliculus was decreased in relative volume. DTI revealed that axial diffusion and fractional anisotropy were reduced in the external capsule and mean diffusion was increased in the fornix, suggesting that restriction of diffusion perpendicular to the axis of the axonal fibers was impaired in these white matter tracts. Therefore, Shank3-deficient rats replicate the reduced brain volume and altered white matter phenotypes present in PMS. Our results indicate that the loss of a glutamatergic synaptic protein, Shank3, has structural consequences at the level of the whole brain. The brain regions that were altered represent potential cross-species structural biomarkers that warrant further study.
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