Complete genome sequence of a novel mitovirus associated with Lagenaria siceraria

Here, we report the discovery and molecular characterization of a novel mitovirus isolated from tissues of Lagenaria siceraria, named Lagenaria siceraria associated mitovirus 1 (LsaMV1). Next-generation sequencing and adapter-ligation-mediated amplification were used to obtain the full-length genome sequence of LsaMV1. The genome of LsaMV1 is 3,098 nucleotides (nt) long and contains an opening reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRP). Homology searches and phylogenetic analysis of the 855-aa RdRP suggested that LsaMV1 is a member of a new species in the family Mitoviridae. This is the first report of a member of the family Mitoviridae associated with the important summer vegetable bottle gourd.

Automated summary

The study reported a novel mitovirus, Lagenaria siceraria associated mitovirus 1 (LsaMV1), isolated from tissues of bottle gourd. The full-length genome sequence of LsaMV1 is 3,098 nucleotides long and encodes a putative RNA-dependent RNA polymerase. Homology searches and phylogenetic analysis indicated that LsaMV1 belongs to a new species in the family Mitoviridae.