4.4 Article

Potential probiotic Lacticaseibacillus rhamnosus MTCC-5897 attenuates Escherichia coli induced inflammatory response in intestinal cells

Journal

ARCHIVES OF MICROBIOLOGY
Volume 203, Issue 9, Pages 5703-5713

Publisher

SPRINGER
DOI: 10.1007/s00203-021-02541-x

Keywords

Probiotic; Intestinal epithelial cells; Cytokines; Immunomodulation; NF-kappa B

Categories

Funding

  1. Department of Biotechnology, Ministry of Science and Technology, New Delhi [BT/PR15109/PFN/20/1174/2015]

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Probiotic Lacticaseibacillus rhamnosus has the potential to prevent gastrointestinal disorders by modulating the immune response and countering inflammatory cytokine expression during exclusion of Escherichia coli in intestinal cells.
Probiotics are microbes having tremendous potential to prevent gastrointestinal disorders. In current investigation, immunomodulatory action of probiotic Lacticaseibacillus rhamnosus MTCC-5897 was studied during exclusion, competition and displacement of Escherichia coli on intestinal epithelial (Caco-2) cells. The incubation of intestinal cells with Escherichia coli, enhanced downstream signalling and activated nuclear factor kappa B (NF-kappa B). This significantly increased (p< 0.01) the pro-inflammatory cytokines (IL-8, TNF-alpha, IFN-gamma) expression. While, incubation of epithelial cells with Lacticaseibacillus rhamnosus during exclusion and competition with Escherichia coli, counteracted these enhanced expressions. The immunomodulatory feature of Lacticaseibacillus rhamnosus was also highlighted with increased (p < 0.05) transcription of toll-like receptor-2 (TLR-2) and single Ig IL-1-related receptor (SIGIRR) along with diminished expression of TLR-4. Likewise, attenuation (p < 0.05) of E. coli-mediated enhanced nuclear translocation of NF-kappa B p-65 subunit by Lacticaseibacillus rhamnosus during exclusion was confirmed with western blotting. Thus, present finding establishes the prophylactic potential of Lacticaseibacillus rhamnosus against exclusion of Escherichia coli in intestinal cells.

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