Methylobrevis albus sp. nov., isolated from freshwater lake sediment

An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22(T), was isolated from sediment of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30 degrees C in a medium containing 0-0.75% (w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C-18:1 omega 7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main respiratory quinone was Q-10. The draft genome sequence of strain L22(T) consisted of 4354,788 bp. The G + C content of genomic DNA was 69.8 mol %. The 16S rRNA gene sequences indicated that strain L22(T) was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to Methylobrevis pamukkalensis JCM 30229(T) with 95.9% 16S rRNA gene sequences similarity. The AAI, ANI and dDDH values between strain L22(T) and M. pamukkalensis JCM 30229(T) were 72.5%, 80.7% and 22.7%. Based on taxonomic results in this study, we proposed that strain L22(T) a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22(T) (=KCTC 72858(T)=MCCC 1H00432(T)).

Automated summary

An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22(T), isolated from sediment of Hulun Lake in Inner Mongolia, China, has been identified as a novel species within the genus Methylobrevis, named Methylobrevis albus sp. nov.