Journal
AQUACULTURE
Volume 539, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.736614
Keywords
Infectious spleen and kidney necrosis virus; Gefitinib; Inhibition; EGFR/PI3K signaling
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Funding
- National Key Research and Development Program of China [2018YFD0900501]
- Natural Science Foundation of Guangdong Province [2020A1515010085]
- China-ASEAN Maritime Cooperation Fund
- Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams [2019KJ140, 2019KJ141]
- Guangdong Basic and Applied Basic Research Foundation [2020B1515120012]
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The study demonstrated that gefitinib could effectively inhibit Infectious spleen and kidney necrosis virus (ISKNV) infection by blocking virus entry and corresponding protein synthesis.
Infectious spleen and kidney necrosis virus disease (ISKNVD) caused significant economic losses to the fishery industry. So it is urgent to develop an effective measure for prevention ISKNVD. In this study, we investigated the effects of gefitinib against ISKNV in vitro and vivo. The gefitinib inhibited viral protein synthesis and decreased viral titers with the dose-dependent manner in Chinese perch brain cells (CPB) cells. Gefitinib was observed to inhibit ISKNV infection by blocking ISKNV entry into CPB cells. Interestingly, gefitinib inhibited EGFR/PI3K phosphorylation and suppressed microfilament gathering induced by ISKNV. At the same time, gefitinib inhibited ISKNV infection and decreased pathogenicity of ISKNV in vivo. These results suggested that gefitinib prevented ISKNV infection by blocking virus-mediated endocytosis. This research provided potential of gefitinib for preventing ISKNVD.
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