Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 105, Issue 13, Pages 5461-5470Publisher
SPRINGER
DOI: 10.1007/s00253-021-11366-z
Keywords
Bacteriophage; Endolysin; Salmonella; Bactericidal activity
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Funding
- National Key Research and Development Program of China [2017YFC1600703]
- Central Public-Interest Scientific Institution Basal Research Fund, YSFRI, CAFS [20603022020004]
- Central Public-Interest Scientific Institution Basal Research Fund, CAFS [2020TD71]
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The recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained in this study, and EDTA was found to enhance its bactericidal activity. LysSP1 showed strong bactericidal activity against Salmonella Typhimurium, exhibited good stability, and demonstrated broad-spectrum bactericidal ability.
Foodborne pathogens have caused many public health incidents and heavy economic burden. Endolysins have been proven to have efficient bactericidal activity against pathogens with low incidence of resistance. In this study, the recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained by prokaryotic expression, and its characteristics were analyzed. Ethylenediaminetetraacetic acid (EDTA) can be used as the outer membrane permeabilizer to increase the bactericidal activity of LysSP1. Under the synergism of 5 mmol/L EDTA, LysSP1 exhibited a strong bactericidal activity against Salmonella Typhimurium ATCC14028. LysSP1 was stable at 4 degrees C for 7 days and at -20 degrees C for 180 days. LysSP1 remained the optimal activity at 40 degrees C and was efficiently active at alkaline condition (pH 8.0-10.0). Divalent metal ions could not enhance the bactericidal activity of LysSP1 and even caused the significant reduction of bactericidal activity. LysSP1 not only could lyse Salmonella, but also could lyse other Gram-negative strains and Gram-positive strains. These results indicated that LysSP1 is a broad-spectrum endolysin and has potential as an antimicrobial agent against Salmonella and other foodborne pathogens.
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