Aegicerativicinus sediminis gen. nov., sp. nov., a novel carotenoid-producing marine bacterium in the family Flavobacteriaceae

A novel bright-yellow pigmented bacterial strain SM2-F-T was isolated from a mangrove sediment collected at the mangrove coast of Luoyang estuary, Quanzhou, China. Strain SM2-F-T was Gram-stain-negative, catalase-weak positive, oxidase-positive, rod-shaped, non-flagellated and non-motile. Growth of strain SM2-F-T was observed at 20-40 degrees C (optimum, 30 degrees C), pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 1.0-4.0% NaCl (optimum, 2.0% NaCl). Flexirubin-pigment was absent, and carotenoid-pigment was present. Phylogenetic analysis of 16S rRNA gene sequence placed strain SM2-F-T into the family Flavobacteriaceae and shared the maximum sequence similarity with Aequorivita soesokkakensis RSSK-12( T) of 92.5%. Whole genomic comparison between strain SM2-F-T and close relatives suggested a novel species of a novel genus. The predominant quinone of strain SM2-F-T was menaquinone (MK)-6. The major fatty acids (> 10%) comprised iso-C-15:1 G (32.4%) and iso-C-15:0 (29.1%). The polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids. The complete genome size was 4,094,245 bp with DNA G + C content of 36.0 mol%. Based on the data of polyphasic study, strain SM2-F-T was considered to represent a novel species of a novel genus, for which the name Aegicerativicinus sediminis gen. nov., sp. nov., was proposed. The type strain was SM2-F-T (= MCCC 1K04383(T) = KCTC 82361( T)).

Automated summary

A novel bright-yellow pigmented bacterial strain SM2-F-T was isolated from mangrove sediment in Quanzhou, China. It belongs to a new genus Aegicerativicinus sediminis gen. nov., sp. nov., with optimal growth at 30 degrees C, pH 7.0, and 2.0% NaCl tolerance.