An Overview of Microcrystal Electron Diffraction (MicroED)

The bedrock of drug discovery and a key tool for understanding cellular function and drug mechanisms of action is the structure determination of chemical compounds, peptides, and proteins. The development of new structure characterization tools, particularly those that fill critical gaps in existing methods, presents important steps forward for structural biology and drug discovery. The emergence of microcrystal electron diffraction (MicroED) expands the application of cryo-electron microscopy to include samples ranging from small molecules and membrane proteins to even large protein complexes using crystals that are one-billionth the size of those required for X-ray crystallography. This review outlines the conception, achievements, and exciting future trajectories for MicroED, an important addition to the existing biophysical toolkit.

Automated summary

The determination of chemical compounds, peptides, and proteins structures plays a crucial role in drug discovery and understanding cellular function. MicroED, with its ability to expand the application of cryo-electron microscopy to samples of various sizes, is an important addition to the existing biophysical toolkit, providing new opportunities for structural biology and drug discovery.