Journal
ANNALS OF BIOMEDICAL ENGINEERING
Volume 49, Issue 12, Pages 3154-3164Publisher
SPRINGER
DOI: 10.1007/s10439-021-02843-7
Keywords
Organ preservation; Kidney preservation; Liver preservation; Heart preservation; Tissue preservation; Machine perfusion; Bioreactor; Closed-loop resuscitation
Categories
Funding
- Department of Defense PRMRP [W81XWH-18-10640]
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The study introduced a novel and portable device that provided oxygenated and pulsatile perfusion, demonstrating its capability to achieve various perfusion parameters under simulated vascular resistance of kidneys, livers, and hearts. The device showed potential value in tissue preservation and physiological studies.
The advantages of oxygenated perfusion are continuing to be demonstrated by many groups focused on improving the efficacy of tissue preservation for transplant, bioreactors for studies of basic tissue physiology, and closed-loop resuscitation. This work presents a novel and portable device that supplies oxygenated and pulsatile perfusion, both of which are regulated by a single pump-oxygenator component comprised of silicone tubes that are cyclically inflated/deflated with compressed oxygen. In this study, pump variables (oxygen supply pressure and length of a silicone tube) were evaluated against hydraulic elements that mimicked the vascular resistance of kidneys, livers, and hearts. The perfusion pressures, flow rates, and oxygenation rates produced by the device were characterized for all configurations of pump variables, and the pulse rates were tuned to improve performance. The device supplied perfusion pressures ranging from 3.5 to 109 mmHg, flow rates ranging from 1.4 to 71.8 mL min(-1), and oxygenation rates up to 316.6 mu mol min(-1). From those results, it was determined that the device was capable of achieving perfusion parameters used in previous kidney, liver, and heart preservation studies. Ultimately, this research demonstrated the efficacy of a novel device that is designed to supply oxygenated perfusion across a range of applications.
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