Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 36, Pages 19614-19619Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202104358
Keywords
calixarene; cell imaging; Forster resonance energy transfer; indicator displacement assay; macrocyclic amphiphile
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Funding
- National Natural Science Foundation of China (NSFC) [21974073, 31961143004]
- NCC Fund [NCC2020FH04]
- Fundamental Research Funds for the Central Universities
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This study presents a method for constructing and optimizing chemical sensors based on supramolecular chemistry principles, addressing the limitations of IDA in bioimaging by combining FRET with IDA, and successfully achieving signal amplification in mitochondria-targeted imaging.
Fluorescent chemosensors are powerful imaging tools in the fields of life sciences and engineering. Based on the principle of supramolecular chemistry, indicator displacement assay (IDA) provides an alternative approach for constructing and optimizing chemosensors, which has the advantages of simplicity, tunability, and modularity. However, the application of IDA in bioimaging continues to face a series of challenges, including interfering signals, background noise, and inconsistent spatial location. Accordingly, we herein report a supramolecular bioimaging strategy of Forster resonance energy transfer (FRET)-assisted IDA by employing macrocyclic amphiphiles as the operating platform. By merging FRET with IDA, the limitations of IDA in bioimaging were addressed. As a proof of concept, the study achieved mitochondria-targeted imaging of adenosine triphosphate in live cells with signal amplification. This study opens a non-covalent avenue for bioimaging with advancements in tunability, generality, and simplicity, apart from the covalent approach.
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